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rabbit antihuman polyclonal antibodies against golph3  (PeproTech)


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    PeproTech rabbit antihuman polyclonal antibodies against golph3
    Expression of miR-3150b was downregulated in HCC cell lines. Relative mRNA expression of miR-3150b normal liver HL7702 cells and four GC cell lines with different differentiation status (MHCC-97L, HepG2, SMMC-7721 and SNU-398) determined by quantitative RT-PCR. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. * p<0.05, ** p<0.01 vs HL7702 cells. <t>GOLPH3,</t> Golgi phosphoprotein 3; HCC, hepatocellular carcinoma.
    Rabbit Antihuman Polyclonal Antibodies Against Golph3, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+antihuman+polyclonal+antibodies+against+golph3/rabbit+antihuman+polyclonal+antibodies+against+golph3/pmc07057801-38-5-17
    Average 90 stars, based on 1 article reviews
    rabbit antihuman polyclonal antibodies against golph3 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3"

    Article Title: MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3

    Journal: Journal of Investigative Medicine

    doi: 10.1136/jim-2019-001181

    Expression of miR-3150b was downregulated in HCC cell lines. Relative mRNA expression of miR-3150b normal liver HL7702 cells and four GC cell lines with different differentiation status (MHCC-97L, HepG2, SMMC-7721 and SNU-398) determined by quantitative RT-PCR. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. * p<0.05, ** p<0.01 vs HL7702 cells. GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma.
    Figure Legend Snippet: Expression of miR-3150b was downregulated in HCC cell lines. Relative mRNA expression of miR-3150b normal liver HL7702 cells and four GC cell lines with different differentiation status (MHCC-97L, HepG2, SMMC-7721 and SNU-398) determined by quantitative RT-PCR. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. * p<0.05, ** p<0.01 vs HL7702 cells. GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma.

    Techniques Used: Expressing, Quantitative RT-PCR

    MiR-3150b suppressed HCC cell proliferation relative miR-3150b expression (A) and CCK-8 cell viability assay (B) in HepG2 cells with transfection of miR-3150b mimic or si-GOLPH3, and in SNU-398 cells transfected with miR-3150b inhibitor or pcDNA3.1-GOLPH3. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. ** p<0.01 vs mimic NC or inhibitor NC group. CCK-8, Cell Counting Kit-8; GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma; NC, negative control.
    Figure Legend Snippet: MiR-3150b suppressed HCC cell proliferation relative miR-3150b expression (A) and CCK-8 cell viability assay (B) in HepG2 cells with transfection of miR-3150b mimic or si-GOLPH3, and in SNU-398 cells transfected with miR-3150b inhibitor or pcDNA3.1-GOLPH3. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. ** p<0.01 vs mimic NC or inhibitor NC group. CCK-8, Cell Counting Kit-8; GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma; NC, negative control.

    Techniques Used: Expressing, CCK-8 Assay, Viability Assay, Transfection, Cell Counting, Negative Control

    GOLPH3 was a direct target of miR-3150b. (A) alignment of miR-3150b with GOLPH3 3'-UTR sequences. HEK293T cells were co-transfected with luciferase reporter with wild-type (WT) GOLPH3 3'-UTR or with mutant GOLPH3 3'-UTR, and miR-3150b or mimic NC for 48 hours. The relative luciferase activity was analyzed by luciferase assay. The mRNA (B) and protein (C) levels of GOLPH3 in HepG2 cells transfected with miR-3150b mimic or mimic NC, and SNU-398 cells transfected with miR-3150b inhibitor or inhibitor NC using quantitative RT-PCR and western blot analysis. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. ** p<0.01, *** p<0.001 vs mimic NC or inhibitor NC group. GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma; NC, negative control; UTR, untranslated region.
    Figure Legend Snippet: GOLPH3 was a direct target of miR-3150b. (A) alignment of miR-3150b with GOLPH3 3'-UTR sequences. HEK293T cells were co-transfected with luciferase reporter with wild-type (WT) GOLPH3 3'-UTR or with mutant GOLPH3 3'-UTR, and miR-3150b or mimic NC for 48 hours. The relative luciferase activity was analyzed by luciferase assay. The mRNA (B) and protein (C) levels of GOLPH3 in HepG2 cells transfected with miR-3150b mimic or mimic NC, and SNU-398 cells transfected with miR-3150b inhibitor or inhibitor NC using quantitative RT-PCR and western blot analysis. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. ** p<0.01, *** p<0.001 vs mimic NC or inhibitor NC group. GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma; NC, negative control; UTR, untranslated region.

    Techniques Used: Transfection, Luciferase, Mutagenesis, Activity Assay, Quantitative RT-PCR, Western Blot, Negative Control

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    Incubation:

    Article Title: MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3
    Article Snippet: .. The blots were incubated with rabbit antihuman polyclonal antibodies against GOLPH3 (catalog no. 19 112–1-AP; 1:2000 dilution; Peprotech, Rocky Hill, New Jersey, USA). ..



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    rabbit antihuman polyclonal antibodies against golph3  (PeproTech)
    90
    PeproTech rabbit antihuman polyclonal antibodies against golph3
    Expression of miR-3150b was downregulated in HCC cell lines. Relative mRNA expression of miR-3150b normal liver HL7702 cells and four GC cell lines with different differentiation status (MHCC-97L, HepG2, SMMC-7721 and SNU-398) determined by quantitative RT-PCR. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. * p<0.05, ** p<0.01 vs HL7702 cells. <t>GOLPH3,</t> Golgi phosphoprotein 3; HCC, hepatocellular carcinoma.
    Rabbit Antihuman Polyclonal Antibodies Against Golph3, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+antihuman+polyclonal+antibodies+against+golph3/rabbit+antihuman+polyclonal+antibodies+against+golph3/pmc07057801-38-5-17
    Average 90 stars, based on 1 article reviews
    rabbit antihuman polyclonal antibodies against golph3 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Expression of miR-3150b was downregulated in HCC cell lines. Relative mRNA expression of miR-3150b normal liver HL7702 cells and four GC cell lines with different differentiation status (MHCC-97L, HepG2, SMMC-7721 and SNU-398) determined by quantitative RT-PCR. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. * p<0.05, ** p<0.01 vs HL7702 cells. GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma.

    Journal: Journal of Investigative Medicine

    Article Title: MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3

    doi: 10.1136/jim-2019-001181

    Figure Lengend Snippet: Expression of miR-3150b was downregulated in HCC cell lines. Relative mRNA expression of miR-3150b normal liver HL7702 cells and four GC cell lines with different differentiation status (MHCC-97L, HepG2, SMMC-7721 and SNU-398) determined by quantitative RT-PCR. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. * p<0.05, ** p<0.01 vs HL7702 cells. GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma.

    Article Snippet: The blots were incubated with rabbit antihuman polyclonal antibodies against GOLPH3 (catalog no. 19 112–1-AP; 1:2000 dilution; Peprotech, Rocky Hill, New Jersey, USA).

    Techniques: Expressing, Quantitative RT-PCR

    MiR-3150b suppressed HCC cell proliferation relative miR-3150b expression (A) and CCK-8 cell viability assay (B) in HepG2 cells with transfection of miR-3150b mimic or si-GOLPH3, and in SNU-398 cells transfected with miR-3150b inhibitor or pcDNA3.1-GOLPH3. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. ** p<0.01 vs mimic NC or inhibitor NC group. CCK-8, Cell Counting Kit-8; GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma; NC, negative control.

    Journal: Journal of Investigative Medicine

    Article Title: MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3

    doi: 10.1136/jim-2019-001181

    Figure Lengend Snippet: MiR-3150b suppressed HCC cell proliferation relative miR-3150b expression (A) and CCK-8 cell viability assay (B) in HepG2 cells with transfection of miR-3150b mimic or si-GOLPH3, and in SNU-398 cells transfected with miR-3150b inhibitor or pcDNA3.1-GOLPH3. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. ** p<0.01 vs mimic NC or inhibitor NC group. CCK-8, Cell Counting Kit-8; GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma; NC, negative control.

    Article Snippet: The blots were incubated with rabbit antihuman polyclonal antibodies against GOLPH3 (catalog no. 19 112–1-AP; 1:2000 dilution; Peprotech, Rocky Hill, New Jersey, USA).

    Techniques: Expressing, CCK-8 Assay, Viability Assay, Transfection, Cell Counting, Negative Control

    GOLPH3 was a direct target of miR-3150b. (A) alignment of miR-3150b with GOLPH3 3'-UTR sequences. HEK293T cells were co-transfected with luciferase reporter with wild-type (WT) GOLPH3 3'-UTR or with mutant GOLPH3 3'-UTR, and miR-3150b or mimic NC for 48 hours. The relative luciferase activity was analyzed by luciferase assay. The mRNA (B) and protein (C) levels of GOLPH3 in HepG2 cells transfected with miR-3150b mimic or mimic NC, and SNU-398 cells transfected with miR-3150b inhibitor or inhibitor NC using quantitative RT-PCR and western blot analysis. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. ** p<0.01, *** p<0.001 vs mimic NC or inhibitor NC group. GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma; NC, negative control; UTR, untranslated region.

    Journal: Journal of Investigative Medicine

    Article Title: MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3

    doi: 10.1136/jim-2019-001181

    Figure Lengend Snippet: GOLPH3 was a direct target of miR-3150b. (A) alignment of miR-3150b with GOLPH3 3'-UTR sequences. HEK293T cells were co-transfected with luciferase reporter with wild-type (WT) GOLPH3 3'-UTR or with mutant GOLPH3 3'-UTR, and miR-3150b or mimic NC for 48 hours. The relative luciferase activity was analyzed by luciferase assay. The mRNA (B) and protein (C) levels of GOLPH3 in HepG2 cells transfected with miR-3150b mimic or mimic NC, and SNU-398 cells transfected with miR-3150b inhibitor or inhibitor NC using quantitative RT-PCR and western blot analysis. All experiments were performed in triplicate with at least three independent experiments. Data were presented as the mean±SD. ** p<0.01, *** p<0.001 vs mimic NC or inhibitor NC group. GOLPH3, Golgi phosphoprotein 3; HCC, hepatocellular carcinoma; NC, negative control; UTR, untranslated region.

    Article Snippet: The blots were incubated with rabbit antihuman polyclonal antibodies against GOLPH3 (catalog no. 19 112–1-AP; 1:2000 dilution; Peprotech, Rocky Hill, New Jersey, USA).

    Techniques: Transfection, Luciferase, Mutagenesis, Activity Assay, Quantitative RT-PCR, Western Blot, Negative Control